{"id":1069,"date":"2016-11-28T18:48:55","date_gmt":"2016-11-28T17:48:55","guid":{"rendered":"http:\/\/www.biobridge-event.com\/knowledge\/?p=1069"},"modified":"2021-05-31T09:04:53","modified_gmt":"2021-05-31T08:04:53","slug":"dr-jolanta-norelli-effect-of-combined-prp-ha-on-bone-marrow-derived-stem-cell-proliferation-in-vitro","status":"publish","type":"post","link":"https:\/\/library.biobridge-event.com\/knowledge\/2016\/11\/28\/dr-jolanta-norelli-effect-of-combined-prp-ha-on-bone-marrow-derived-stem-cell-proliferation-in-vitro\/","title":{"rendered":"Dr. Jolanta Norelli &#8211; Effect of combined PRP-HA on bone marrow-derived stem cell proliferation in vitro"},"content":{"rendered":"<div class=\"video-shortcode\">\n<div class=\"embed-vimeo\" style=\"text-align: center;\"><iframe src=\"https:\/\/player.vimeo.com\/video\/192934424\" width=\"1100\" height=\"619\" frameborder=\"0\" webkitallowfullscreen mozallowfullscreen allowfullscreen><\/iframe><\/div>\n<\/div>\n<p>Purpose<br \/>\nInvestigating the effects of Platelet Rich Plasma (PRP) and Hyaluronic Acid (HA) on bone marrow-derived stem cells (BMSCs) proliferation.<\/p>\n<p>Methods<br \/>\nSprague-Dawley rat (SDR) blood centrifuged via RegenKit\u00ae protocols in BCT tubes produced 4-5 ml PRP with 1.6-fold platelet count increase and &gt;85% WBC depletion. CaCl2 activation (100 nM) of pooled PRP prevented gelling reaction with media ensuring nutrient diffusion to cells.<br \/>\nSDR femur BMSCs were cultured (DMEM\/low glucose media, 1% antibiotic\/antimycotic, 10% FBS) until P4 and plated at 3.6 x 104 cells\/well.<br \/>\nTwelve experimental media contained PRP at 1%, 5%, 10%, 20%, HA at 0.25 mg, 0.5 mg, 0.75 mg, and 1.0 mg, 1% PRP + 0.25 mg or 0.5 mg HA (1550 kDA non-crosslinked, Regen Lab SA), and 5% PRP + 0.25 mg or 0.5 mg HA. Samples were prepared in quadruplicate with media changes at days 3 and 6.<br \/>\nCellular proliferation was measured with MTS formazan assay and Mann-Whitney U nonparametric tests for statistical analysis.<\/p>\n<p>Results<br \/>\nSignificant increases in BMSC proliferation were observed with 1% (p = 0.0006), 5% (p = 0.0059), 10% (p = 0.006), and 20% (p = 0.0015) PRP but not with HA. A trend across PRP groups (p = 0.007) suggests dose-response effects.<\/p>\n<p>Conclusion<br \/>\nWe observed a significant increase in BMSC proliferation in culture with PRP and a negative proliferative trend at high PRP concentrations,possibly from cell surface receptor downregulation. It is unclear why enhanced proliferation was not observed with HA.<br \/>\nWhether use of rat blood in a system designed for human blood affects PRP preparations remains unknown. Our research coordinates with a multi-center placebo-controlled RCT comparing the effects of intraarticular PRP-HA and HA-alone knee supplementation in patients who previously received intraarticular HA but continue to have knee pain.<\/p>\n","protected":false},"excerpt":{"rendered":"<p> [&hellip;]<\/p>\n","protected":false},"author":6,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_bbp_topic_count":0,"_bbp_reply_count":0,"_bbp_total_topic_count":0,"_bbp_total_reply_count":0,"_bbp_voice_count":0,"_bbp_anonymous_reply_count":0,"_bbp_topic_count_hidden":0,"_bbp_reply_count_hidden":0,"_bbp_forum_subforum_count":0,"_mi_skip_tracking":false,"_exactmetrics_sitenote_active":false,"_exactmetrics_sitenote_note":"","_exactmetrics_sitenote_category":0,"_price":"","_stock":"","_tribe_ticket_header":"","_tribe_default_ticket_provider":"","_tribe_ticket_capacity":"0","_ticket_start_date":"","_ticket_end_date":"","_tribe_ticket_show_description":"","_tribe_ticket_show_not_going":false,"_tribe_ticket_use_global_stock":"","_tribe_ticket_global_stock_level":"","_global_stock_mode":"","_global_stock_cap":"","_tribe_rsvp_for_event":"","_tribe_ticket_going_count":"","_tribe_ticket_not_going_count":"","_tribe_tickets_list":[],"_tribe_ticket_has_attendee_info_fields":false,"spay_email":""},"categories":[8,59],"tags":[12,4,30],"aioseo_notices":[],"jetpack_featured_media_url":"","jetpack_shortlink":"https:\/\/wp.me\/pawNNh-hf","jetpack_likes_enabled":true,"_links":{"self":[{"href":"https:\/\/library.biobridge-event.com\/knowledge\/wp-json\/wp\/v2\/posts\/1069"}],"collection":[{"href":"https:\/\/library.biobridge-event.com\/knowledge\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/library.biobridge-event.com\/knowledge\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/library.biobridge-event.com\/knowledge\/wp-json\/wp\/v2\/users\/6"}],"replies":[{"embeddable":true,"href":"https:\/\/library.biobridge-event.com\/knowledge\/wp-json\/wp\/v2\/comments?post=1069"}],"version-history":[{"count":1,"href":"https:\/\/library.biobridge-event.com\/knowledge\/wp-json\/wp\/v2\/posts\/1069\/revisions"}],"predecessor-version":[{"id":3466,"href":"https:\/\/library.biobridge-event.com\/knowledge\/wp-json\/wp\/v2\/posts\/1069\/revisions\/3466"}],"wp:attachment":[{"href":"https:\/\/library.biobridge-event.com\/knowledge\/wp-json\/wp\/v2\/media?parent=1069"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/library.biobridge-event.com\/knowledge\/wp-json\/wp\/v2\/categories?post=1069"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/library.biobridge-event.com\/knowledge\/wp-json\/wp\/v2\/tags?post=1069"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}