Background
Mesenchymal stem cells derived from adipose tissue (AT-MSCs) are promising candidates for cell therapy and tissue engineering strategies. Currently the use of non-autologous cell culture media (e.g animal-derived or allogenic serum) for clinical applications of mesenchymal stem cells is criticized by regulatory agencies. To make Clinical translation of Mesenchymal stem cells safer and more efficient, here we propose to substitute actual cell culture media by autologous platelet-rich-plasma (PRP) as a safer alternative medium supplement for AT-MSC culture.

Methods
AT-MSCs were cultured for 10 days in media supplemented with different concentrations of autologous PRP (1%-60%) prepared with RegenLab kit. AT-MSC proliferation, cell phenotype and multipotency capacity were assessed and compared to AT-MSCs expanded in a classical medium supplemented with 10% of fetal bovine serum (FBS).

Results
Culture media supplemented with PRP showed dose-dependent higher AT-MSC proliferation than did FBS. 20% PRP was the most effective concentration to promote cell proliferation. This condition increased 13.9 times AT-MSC number in comparison to culture with FBS, without changing the AT-MSC phenotype and differentiation capacity. 57% of platelets were viable up during 10 days of culture and secreted main growth factors needed for MSCs proliferation.

Conclusion
We concluded that 20% autologous PRP is a safe, efficient and cost-effective supplement for AT-MSC expansion. It should be considered as an alternative to FBS or other non-autologous blood derivatives. It could serve as a potent substitute for the validation of future clinical protocols even for other stem cells type, as it respects good-manufacturing practices and regulatory agencies standards.

How PRP can improve fat grafting?
The transplant of autologous fat tissue, so called fast grafting, lipomodelage, liposculpture or lipofilling is known since the beginning of 20th century.

The principle of the technique is to transfer patient’s own fat tissue from a donor site to a site where it misses volume. Its first indications were for aesthetic surgery of the face, and then for hands. Fat grafting is also useful for tissue loss due to an accident, operation, congenital disease or lipodystrophy. In addition to a volumizing effect, the injected fat leads to a neoangiogenesis effect improving the cutaneous trophicity, and to an antiaging effect. This technique is thus also recommended for wound healing, scar reduction radiodermitis treatment and correction of acne scars. During the last decade, fat grafting has been used more and more frequently for breast reconstruction and augmentation.

The survival rate and longstanding results varying in the literature between 10 to 90% depend on indications and on surgical technique. Different techniques have been proposed to improve the survival rate of fat grafting and its predictability. At present, the most used method for the fat harvesting, purification and infiltration is the one described in detail by Coleman in 1986. Here we expose a new technique of fat grafting with PRP and illustrate its indications to obtain the best satisfaction for both patients and doctors.